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KMID : 0545120100200071128
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 7 p.1128 ~ p.1133
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Diversity and Abundance of Ammonia-Oxidizing Bacteria in Activated Sludge Treating Different Types of Wastewater
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Baek Kyung-Hwa
Park Chul
Oh Hee-Mock
Yoon Byung-Dae
Kim Hee-Sik
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Abstract
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The diversity and abundance of ammonia-oxidizing bacteria (AOB) in activated sludge were compared using PCR-DGGE and real-time PCR assays. Activated sludge samples were collected from five different types of wastewater treatment plants (WWTP) mainly treating textile, paper, food and livestock wastewater or domestic sewage. The composition of total bacteria determined by PCR-DGGE was highly diverse between the samples while the community of AOB was similar across all the investigated activated sludge. Total bacterial numbers and AOB numbers in the aerated mixed liquor were in the range of 1.8×1010 to 3.8×1012 and 1.7×106 to 2.7×1010 copies/l, respectively. Activated sludge from livestock, textile, and sewage treating WWTP contained relatively high amoA gene copies (more than 105 copies/l) while activated sludge from food and paper WWTP revealed the low number of amoA gene (less than 103 copies/L). The value of the amoA gene copy effectively showed the difference in composition of bacteria in different activated sludge samples and this was better than the measurement with the AOB 16S rRNA or total 16S rRNA gene. These results suggest that the quantification of the amoA gene can help monitor AOB and ammonia oxidation in WWTP.
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KEYWORD
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ammonia-oxidizing bacteria, activated sludge, PCR-DGGE, real-time PCR, wastewater
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